blot n. 1.墨污,墨漬,污點(diǎn),污斑。 2.瑕疵;恥辱,污名。 3.〔古語(yǔ)〕涂去,抹去。 a blot on her past [her character] 她歷史[品格]上的污點(diǎn)。 drop an ink blot on an envelope 信封玷上了一塊墨污。 vt. (-tt-) 1.用(墨水等)弄臟,涂污。 2.涂去,抹掉(out)。 3.用吸墨紙吸干。 4.遮蔽(風(fēng)景等),遮暗,使昏暗。 5.玷污,損害(名譽(yù)等)。 6.〔美俚〕殺死;亂涂,亂寫(xiě);〔詩(shī)〕擦掉。 The sun was blotted by the moon. 月亮遮暗了太陽(yáng),日蝕。 blot the wet pane 擦干潮濕的窗玻璃。 blotting pad 吸墨紙滾臺(tái)。 vi. 1.(墨水)滲開(kāi);造成污漬。 2.(紙等)玷上墨污;(吸墨紙)吸掉墨水。 This pen blots. 這枝筆漏墨水。 This paper blots easily. 這種紙容易吸水。 blot one's copybook 損壞自己名譽(yù)。 blot out 1. 涂去(文字)。 2. 遮掉(風(fēng)景等),遮暗。 3. 〔美俚〕消滅,殺掉;毀掉。 n. 1.(十五子棋中)易被吃掉的孤立棋子。 2.弱點(diǎn)。 hit a blot 吃去弱子,突破弱點(diǎn)。
Bl21 . the dot blot hybridization verified that the scfv was secreted to the medium 從培養(yǎng)基中可檢測(cè)到可溶性抗人絨毛膜促性腺激素的單鏈抗體。
The analysis results of southern blot hybridization among all of specimens are coincidence with the detecting results of pcr products electrophoresis 所有標(biāo)本進(jìn)行southernblot雜交分析,結(jié)果予以證實(shí)。
Northern blot hybridization showed that leacbf expression had the same tendency with the enzyme activity . and it showed that the transcription factor acbf maybe have some relation with the enzyme activity of pal 表明在紫外光照射條件下, leacbf編碼的轉(zhuǎn)錄因子的變化趨勢(shì)與苯丙氨酸解氨酶( pal )活性的變化趨勢(shì)有一定的相關(guān)性。
Northern blot hybridization showed that leacbf has well - distributed expression in different tissues of wild tomato ( pink stage ) . treatment the seedling with uv made the enzyme activity of the pal increased gradually and then decreased 用不同的紫外線照射時(shí)間處理番茄幼芽,苯丙氨酸解氨酶( pal )的活性呈先是增加,達(dá)到最大值后再減小的趨勢(shì)。
Northern blot hybridization showed that leerfl , leerf2 and pti4 ( a member of erebps in tomato ) expressed differentially in different parts of wild tomato fruit during ripening , which was related with fruit ripening Northern雜交分析表明: leerf1 、 leerf2 、 pti4 (已發(fā)表的番茄erebps成員)基因在普通番茄果實(shí)的成熟過(guò)程及果實(shí)的不同部位都有差異表達(dá),表現(xiàn)出與成熟進(jìn)程的相關(guān)性。
Lastly by using the technique of dot blot hybridization , the genome dna of chlamydia was detected with the probe of momp gene labeled with dig - 11 - dutp by using the way of random primer . the results showed the degree of sensitivity of the probe was 10 pg and other pathogens could not be detected by this probe . by comparing the diagnostic ways of nucleotide probe and fc , the technique of nucleotide probe were proved to have high sensitivity and speci fi city 最后,用地高辛隨機(jī)引物法標(biāo)記成momp基因核酸探針,斑點(diǎn)雜交檢測(cè)衣原體基因組dna ,靈敏度可達(dá)10pg ,且不能檢出其它病原體的核酸。將核酸探針?lè)ㄅc補(bǔ)體結(jié)合反應(yīng)法對(duì)衣原體感染的診斷進(jìn)行比較,初步證明該探針具有較高的敏感性與較強(qiáng)的特異性。
Molecular examination and biological observation of transformed plants by growing resistant germinations we got 30 transformed plants . pcr and spot blotting hybridization analysis indicate that hsp70 anti - sense cdna had been integrated into tobacco genome . through observation and measurement we found 12 completely sterile , 9hyper - sterile , 3 partially sterile and 6 fertile and significant differences between them in stamen , height , and fruit weight Pcr和點(diǎn)雜交分析表明, hsp70正、反義。 dna己經(jīng)整合到煙草的基因組中。在進(jìn)行田間生物學(xué)性狀觀察時(shí), 30株轉(zhuǎn)基因植株中有12株表現(xiàn)出完全不育, 9株高度不育, 3株部分不育和6株可育;不育植株在雄蕊、株高和果實(shí)重量等方面都存在明顯的差異。
Fingerprints of 5 strains of the inbred mice and 2 strains of the inbred rats was conducted using a nonisotopically hrp labeled jl - 02 by the second institute of the public safety bureau of china and southern blot hybridization , the author studied many fingerprints of the same dna , the different organic fingerprints of the same organism and fingerprints of parent and offspring . the patterns were completely different among the different strains and those of the samples from the same strain were completely identical 采用公安部二所自行研制的jl - 02多位點(diǎn)探針對(duì)5個(gè)品系的近交系小鼠和2個(gè)品系近交系大鼠進(jìn)行了dna指紋分析,經(jīng)過(guò)對(duì)同一dna的反復(fù)制作dna指紋圖和同一個(gè)體不同組織進(jìn)行的dna指紋圖制作及對(duì)親代和子代(同品系內(nèi)和不同品系間雜交)間的dna指紋圖比較。
The engineering bacterium which carried bcih i - chi and i - glu cdna was pcg - ii . two methods of agrobacterium - mediated and gene gun were used to transformate long ya lillium . the results of pcr analysis and southern dot blotting hybridization demonstrated that the chi a nd glu cdna have been intergrated into host genome . at the same time ; compared agrabactenum - mediated method with gene gun method , the transformation frequency of the former was 16 . 7 % , while the latter was 50 % , so gene gun transformation method was suitable for long ya liiliwn 用攜帶有幾丁質(zhì)酶基因和- 1 、 3葡聚糖酶基因的工程菌,通過(guò)農(nóng)桿菌介導(dǎo)法和基因槍轉(zhuǎn)化法轉(zhuǎn)化龍牙百合,經(jīng)pcr和點(diǎn)雜交檢測(cè)證明外源基因已經(jīng)整合到植物染色體中。同時(shí)對(duì)農(nóng)桿菌介導(dǎo)法和基因槍法進(jìn)行比較,發(fā)現(xiàn)農(nóng)桿菌介導(dǎo)法的轉(zhuǎn)化率為16 . 7 ,基因槍法的轉(zhuǎn)化率為50 ,因此可能基因槍轉(zhuǎn)化法更適于龍牙百合的遺傳轉(zhuǎn)化。
The more accurate localization of 3galt - l mrna expression in mouse brain as studied by in situ hybridization was in good agreement with the general expression pattern seen with northern blot hybridization . in newborn mice , the dense granular layer and the purkinje cell showed a strong signal 我們發(fā)現(xiàn)在小腦中1型鏈結(jié)構(gòu)的變化與邵galt一1的表達(dá)變化基本一致:在新生鼠小腦中, 1型鏈結(jié)構(gòu)在蒲肯野細(xì)胞和顆粒層細(xì)胞中都有表達(dá),而在成年鼠小腦中, 1型鏈結(jié)構(gòu)僅在蒲肯野細(xì)胞中表達(dá)。